Induced differentiation of human bone marrow mesenchymal stem cells towards neuron-like cells
10.3969/j.issn.1673-8225.2009.27.039
- VernacularTitle:人骨髓间充质干细胞向神经元样细胞诱导分化
- Author:
Suiyi XU
;
Guanglai LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(27):5385-5388
- CountryChina
- Language:Chinese
-
Abstract:
Bone marrow mesenchymal stem cell (BMSC) provides the merits such as easy to obtain and small injury. Its low immunogenicity and its potential to easily induce immune tolerance make allogeneic transplant feasible without human leucocyte antigen matching to reduce the adverse effects of immunosupprassants. Density gradient centrifugation has been mostly used to isolate and culture BMSCs in vitro, which can be identified according to biological characteristics, cell surface markers, multi-differentiation potential, low immunogenicity and immune regulation. BMSCs are form the mesoderm, but they can be induced towards the calls from endoderm or ectoderm. Generally, BMSCs of the fifth passage are selected. In addition to use of conventional inducing agents, such as nerve growth factors, ratinoic acid, brain-derived growth factors, basic fibroblast growth factors, and epidermal growth factors, docesahexenoic acid and arachidonic acid are added to accelerate the neuron-like cell differentiation of BMSCs and concomitantly to promote the growth of neuronal axon. However, the security of BMSCs transplantation, in particular the tumorigenicity of tissue, requires further investigation.
