Effect of epidemic growth factor combining with basic fibroblast growth factor on wound healing of penetrative keratic autoplasy in rabbits
- VernacularTitle:表皮细胞生长因子和碱性成纤维细胞生长因子联合点眼对兔自体穿透性角膜移植伤口愈合的影响
- Author:
Chao DAI
;
Xiang LIU
;
Laihao WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Trauma
2001;17(6):363-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of epidemic growth factor (EGF) combining with basic fibroblast growth factor (bFGF) on wound healing of the penetrative keratic autoplasy in rabbits. Methods All 18 rabbits (36 eyes) were randomly divided into 9 groups and undergone penetrative keratic autoplasy, eyedrop.Diameters of the trephines were all 6 mm. Liquid scintillation counter was used to measure the incorporation rate of 3H-TdR. Pressure-detector was used to survey the intensity of keratic wound healing. AgNORs staining was used to count the fibroblast cells at keratic wound. VG staining, AgNORs staining and transmission electron microscopy were used to observe the morphological changes of keratic wound. Results (1) Both the wound intensity and the number of fibroblast cells of combination groups were higher than the 2 control groups in 8 days. (2) From the 14 th day to 21 st day, the wound intensity, the number of fibroblast cells and the incorporation rate of 3H-TdR in wound healing had no obvious difference from the positive control groups. Conclusions At the primary time, EGF combining with bFGF topic is more efficient to accelerate the wound healing of PKP. But the effect of this combination topic is just the same as bFGF topic alone 14 days later.
