Application of chitosan-gold nanoparticles hybrid film biosensor in glucose measurement
10.3969/j.issn.1673-8225.2009.29.044
- VernacularTitle:壳聚糖和纳米金混合膜生物传感器在葡萄糖检测中的应用
- Author:
Guoquan WU
;
Zhongyan LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(29):5789-5792
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:At present,syndromes happening frequency of diabetes mellitus can be controlled through regulating glucose concentration in blood.Therefore,a fast,steady,accurate glucose biosensor has been an intense area of focus for investigators.OBJECTIVE:To observe the effect of chitosan-gold nanoparticles hybrid film on electrochemical glucose biosensor.DESIGN,TIME AND SETTING:The observation experiment was performed at the Department of Medicine Laboratory,General Hospital,Guangzhou Military Command of Chinese PLA from February to April 2009.MATERIALS:Based on the immobilization of glucose oxidase (GOD) with cross-linking in the matrix of chitosan on an Au electrode by glutaraldehyde,a novel glucose biosensor was constructed with gold nanoparticles by electrodeposition.METHODS:The properties of CHIT/AuNPs hybrid film modified electrode were characterized by cyclic voltammetry (CV) in 20 mmol/L K3Fe (CN)6-0.1 mol/L KCI system by using the three-electrode system and the response to glucose was investigated by linear scan voltammetry (LSV).The optimal experiment conditions,stability and reproducibility about the glucose biosensor were also studied.MAIN OUTCOME MEASURES:The cyclic voltammetry properties of different modified electrode,as well as relation between glucose concentration and response current.RESULTS:Glucose concentration showed a linear correlation with response current in range of 0.001-6 mmol/L.The biosensor exhibited excellent performances of producibility,stability and the ignorance interferences of reducing substances,with the CONCLUSION:The glucose biosensor possesses excellent reproducibility and stability with a fast response time to glucose,which can be used in detection of glucose concentration in human serum.
