ThyminE-Hg2+-Thymine StructurE-based Detection of Hg2+ Enhanced by Gold Nanoparticle
- VernacularTitle:基于胸腺嘧啶-汞离子-胸腺嘧啶结构和纳米金放大传感器检测汞离子
- Author:
Zhihong MO
;
Linling YANG
;
Xiaochao YANG
;
Zifeng CHEN
- Publication Type:Journal Article
- Keywords:
Mercury ion;
oligonucleotide;
gold nanoparticle;
quartz crystal microbalance
- From:
Chinese Journal of Analytical Chemistry
2009;37(7):1033-1036
- CountryChina
- Language:Chinese
-
Abstract:
A simple and sensitive method was developed for the detection of mercury ions with quartz crystal microbalance(QCM), based on the specific thymine(T)-Hg2+-T structure and gold nanoparticles. The gold nanoparticles were prepared by the citrate reduction method. In a self-assembled way, the particle surface was modified with the probe oligonucleotides which were partially replaced by 6-Mercaptohexan-1-ol to reduce the steric hindrance of hybridization. The sensitivity was optimized by the oligonucleotide with a strand length of 9bp and a T number of 7. The linear range was 5.0-100 nmol/L with a detection limit of 2.0 nmol/L. And Ca2+, Mg2+ and other metal ions had no significant interferences. This method was successfully applied for the detection of Hg2+in environmental water samples, with RSD less than 2.9% and recoveries of 97.3%-101.2%.
