Influence of quercetin and bornyl acetate on ratio of IFN-γ/IL-4 and macrophages in uteri of LPS-induced abortion mice
10.3969/j.issn.0529-1356.2009.04.023
- VernacularTitle:槲皮素和乙酸龙脑酯对细菌脂多糖诱导流产小鼠子宫组织IFN-γ/IL-4和巨噬细胞的影响
- Author:
Xiaodan WANG
;
Aituan MA
;
Wanyu SHI
;
Guojun JIANG
;
Xiuhui ZHONG
- Publication Type:Journal Article
- Keywords:
Embryo resorption;
Lipopolysaccharide;
Macrophage;
IFN-γ;
IL-4;
Immunohistochemistry;
Mouse
- From:
Acta Anatomica Sinica
2009;40(4):637-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate significance of interferon-γ/interleukin-4(IFN-γ/IL-4) and macrophages in uterus in early embryo loss (or resorption), and to elucidate the anti-abortive effect and the immunological modulation at maternal-fetal interface with quercetin and bornvl acetate. Methods Lipopolysaccharide (LPS)(0.10μg/mouse)was injected via tail vein in order to induce abortion in 7-day-gestation mice which received quercetin and bornvl acetate at days 4-7 of gestation. Levels of IFN-γ and IL-4 in uterus lysate supernatant were measured using enzyme-linked immuno-absorbent assay (ELISA), and uterine macrophages of each group ( n =10) were detected by immunohistochemistry. Results The amount of macrophages was much higher in the uteri of LPS-induced abortion mice than that in control mice. The abortion rate of mouse declined to certain level. The therapy of quercetin combined with bornyl acetate reversed LPS-induced abortion. Conclusion The increase of IFN-γ/IL-4 and the amount of macrophages in the LPS-treated mouse uterus is associated with the embryo loss, and quercetin and bornyl acetate have the anti-abortive effect through modulation of maternal-fetal interface immunity balance.
