Compararative investigation of proliferation of lymphocytes stimulated by human umbilical cord blood mesenchymal stem cells with CFSE and MTT
10.3969/j.issn.0529-1356.2009.04.032
- VernacularTitle:羧基荧光素乙酰乙酸与四甲基偶氮唑盐法检测人脐血间充质干细胞刺激脾淋巴细胞增殖作用的比较
- Author:
Haimei SUN
;
Fengqing JI
;
Yi WANG
;
Danni WANG
;
Chunli ZHAO
;
Hui YANG
- Publication Type:Journal Article
- Keywords:
Umbilical cord blood mesenchymal stem cell;
Lymphocyte;
Proliferation;
Carboxyfluorescein didcetate;
MTT assay
- From:
Acta Anatomica Sinica
2009;40(4):680-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the sensitivities of the stimulation effect of human cord blood mesenchymal stem cells(CB-MSCs) and CB-MSCs of neuronal differentiation to lymphocytes(LCs) detected with carboxyfluorescein didcetate(CFSE) and MTT. Methods To prepare LCs from SD rat and divided into four group stimulating cells: 1. CB-MSCs;2. Dif-CB-HSCs;3. SH-SY5Y(positive control);4. Auto-LC(negative control).Stimulating cells were respectively Co-cultured with LCs. The proliferation of LCs was detected with MTT and CFSE ( n =3). Results CB-MSCs and Dif-CB-HSCs stimulated LCs to proliferate more weakly than positive control detected with MTT and CFSE. The quantity of proliferation of lymphocytes Co-cultured with CB-MSCs and Dif-CB-HSCs were higher than that of Auto-LC detected with CFSE. But MTT OD value of CB-MSCs and Dif-CB-HSCs was a little lower than that of Auto-LC. Statistical analysis results showed no significant difference.Conclusion CFSE can reflect proliferation status of lymphocytes better than MTT. CFSE shows more advantages in practical use.
