Effects of Simvastatin on the proliferation and osteoblastic differentiation of human periodontal ligament cells

VernacularTitle:辛伐他汀对人牙周膜细胞增殖和分化的影响
Author: Fei HU ; Xueyang ZHANG ; Lei ZHOU
Publication Type:Journal Article
Keywords: Simvastatin; Periodontal ligament cells; Proliferation; Osteoblastic
From: Journal of Practical Stomatology 2009;25(4):505-508
CountryChina
Language:Chinese
Abstract: Objective: To study the effects of Simvastatin on the proliferation and osteoblastic differentiation of human periodontal ligament (PDL) cells. Methods: The fourth passage human PDLs were cultured in conditioned mineralization medium with different concentrations of Simvastatin. Proliferation ability was detected by MTT method. Alkaline phosphatase (ALP) activity was measured with 4-nitrophenyl phosphate hexahydrate (PNPP) assay. Results: Proliferation and osteoblastic differentiation of human PDLs were increased in all treatment groups with different concentrations of Simvastatin(10-9, 10-8, 10-7, 10-6 mol/L). Compared with control group, significant statistical difference was found in 10-8 mol/L, 10-7 mol/L and 10-6 mol/L groups(P<0.05). The maximum effect was observed at the concentration of 10-7 mol/L. Conclusion: Optimal concentration of Simvastatin can improve the proliferation and osteoblastic differentiation of human PDLs.