A primary study of the different protein expression profiles between human oral squamous cell carcinoma and normal oral mucosa tissues
10.3969/j.issn.1001-3733.2009.04.020
- VernacularTitle:口腔鳞癌组织与口腔正常黏膜组织蛋白质差异表达的初步研究
- Author:
Wenmei WANG
;
Chunlan ZHENG
;
Qingang HU
;
Wenhui JIANG
;
Xiaofeng HUANG
;
Wei TANG
- Publication Type:Journal Article
- Keywords:
Mouth;
Squamous cell carcinoma(OSCC);
Two-dimensional gel electrophoresis;
Proteome;
Peptide mass finger print(PMF)
- From:
Journal of Practical Stomatology
2009;25(4):540-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the different protein expression profiles between human oral squamous cell carcinoma (OSCC) and normal oral mucosa tissues, and provide experimental data for further study of the development mechanism of OSCC. Methods: 10 cases of OSCC and paired normal oral mucosa tissues were collected and analyzed through two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Results: (1) The average protein spots of OSCC were 2 325±390, while that of normal oral mucosa tissues were 2 487±281. (2) 29 differential protein spots were found between OSCC and normal oral mucosa. Moreover, these protein spots were all down- regulated in OSCC compared with normal oral mucosa. Among these spots, 3 were identified as fibrin beta, triosephosphate isomerase (TIM) and unknown protein through mass spectrometry and bioinformation. Conclusion: Down-regulation of fibrin beta, Triosephosphate isomerase(TIM) and unknown protein are found in the development of OSCC and the mechanism needs further study.
