Different gene expressions in human periodontal cells after being treated with Emdogain
10.3969/j.issn.1001-3733.2009.04.024
- VernacularTitle:Emdogain作用下人牙周膜细胞的差异表达基因
- Author:
Xueyang ZHANG
;
Hua ZHAO
;
Jincai ZHANG
- Publication Type:Journal Article
- Keywords:
Emdogain;
Periodontal cells;
Differentially expressed genes
- From:
Journal of Practical Stomatology
2009;25(4):556-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the different gene expressions in human periodontal cells after being treated with Emdogain(EMD). Methods: The fourth passage periodontal cells were cultured. Cells in experimental group were cultured with 100 μg/ml EMD in non-serum DMEM for 3 days. Cells in control group were cultured in non-serum DMEM for 3 days. The gene expressions in human periodontal cells were screened with gene chip technique. Results: There were 112 differently expressed genes, 11 down-regulated genes and 101 up-regulated genes. Conclusion: After treated with Emdogain, expression of genes related to cell growth, cell proliferation, biosynthesis of extracellular matrix, angiogenesis and osteogenesis is up-regulated dramatically. Genes related to lipid metabolization and keratinocyte growth is down-regulated in human periodontal cells.
