Effect of ginsenoside Rb1 on N9 cell activation induced by oxygen deficit
10.3969/j.issn.0529-1356.2009.04.004
- VernacularTitle:人参皂苷Rb1对缺氧诱导N9细胞应激活化反应的影响
- Author:
Lining KE
;
Wei WANG
;
Jianwen XU
;
Jianyin LIN
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb1;
Microglia N9;
Hypoxic;
Activation;
Immunofluorescence
- From:
Acta Anatomica Sinica
2009;40(4):533-538
- CountryChina
- Language:Chinese
-
Abstract:
Objective To activate microglia N9 cell through the oxygen deficit, and to discuss the influence to the N9 cell by ginsenoside Rb1, laying the foundation for the basic study and the clinical medicine development. Methods Through ginsenoside Rb1 intervention, the cell morphology the proliferation ability were observed, ELISA, fluorescent probe DAF-FM DA, Griess the reagent examination, were used to measure TNF-α, the O-2 output, the NO content change, chemiluminescence, the immunofluorescence method, and plastochondria membrane potential, were carried out to detect the cytochrome C content. Results Regardless of being preventive or medical gives, ginsenoside Rb1 can decline the NO,O-2,TNF-α high expression; and reduce the plastochondria membrane potential changing, the cytochrome C redistribution. Conclusion Ginsenoside Rb1 can decline N9 cell activation to a certain extent, reduce expression of the nerve toxic factor, and to stabilize mitochondrial membrane potential and distribution of cytochrome C.
