Human bone marrow mesenchymal stem cells could beinduced to differentiation by low dose sodium arsnite
10.3969/j.issn.0529-1356.2009.04.012
- VernacularTitle:长期低剂量NaAsO2诱导人骨髓间充质干细胞的分化
- Author:
Lina FENG
;
Yi WANG
;
Siyuan LI
;
Xiaoling MU
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
arsenite resistance cells;
Differentiation;
Oct-4 ABCG2;
RT-PCR;
Human
- From:
Acta Anatomica Sinica
2009;40(4):580-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of low dose sodium arsenite to human bone marrow mesenchymal stem cells(hBMSCs) differentiation during establishing of arsenic-resistant cell model. Methods hBMSCs were prepared in conventional method and continuously exposed to 1μmol/L sodium arsenite for ≥12weeks inv vitro. Forty-eight hours acute arsenite toxicity test was drived to assay if the cells acquired arsenic-resistance. The proliferation capacity of CAsE-hBMSCs was observed by the rate of colony formation.The expression of Oct-4 in CAsE-hBMSCs was assayed by RT-PCR and immunocytochemistry. The expression of ABCG2 in CAsE-hBMSCs was analyzed by RT-PCR. Results hBMSCs continuously exposed to 1μmol/L sodium asenite for ≥12 weeks exhibited dramatic resistance to acute arsenite toxicity. The LC 50 for acute arsenite exposure in CAsE-hBMSCs was 35.59μmol/L versus 18.04μmol/L in control cells. Compared to control cells, the CAsE-hBMSCs didn't show malignant proliferation ability. Expression of Oct-4 gene was positive in 4th, 18th passage hBMSCs and the hBMSCs induced by arsenite for 4 weeks but negative in CAsE-hBMSCs. The expression of Oct-4 protein was positive and weakly positive in 4th passage hBMSCs and CAsE-hBMSCs respectively, and the positive granules of Oct-4 distributed in cytoplasm. The expression of ABCG2 gene in CAsE-hBMSCs was obviously lower than that in control cells ( P <0.001). Conclusion Human bone marrow mesenchymal stem cells could be induced to differentiation by low dose sodium arsenite.
