Effect of Grp75 on the alteration of apoptosis related gene Bax and NF-κB induced by glucose deprivation
10.3969/j.issn.0529-1356.2009.04.00
- VernacularTitle:葡萄糖调节蛋白75对缺糖诱导的凋亡相关基因Bax和NF-κB改变的影响
- Author:
Ling YANG
;
Mingxia ZHAO
;
Wen LIU
;
Xiaoyu LIU
;
Jinyu HAO
;
Ji ZUO
- Publication Type:Journal Article
- Keywords:
Glucose regulated protein 75;
Bax;
NF-κB;
Glucose deprivation;
Western blotting
- From:
Acta Anatomica Sinica
2009;40(4):594-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of glucose regulated protein 75(Grp75) on the alteration of Bax and NF-κB induced by glucose deprivation through the stably transfected PC12 cells with Grp75. Methods The cells of Grp75-overexpressing group and control group incubated in glucose-free DMEM medium for indicated time (6, 12, 24 and 48hours). The expression level of Grp75, Bax and the activity of NF-κB were determined by Western blotting, and the expression level of Bax was determined by semi-quantitative RT-PCR and Western blotting. Immunocytochemistry was performed using a conformation specific anti-Bax (6A7) antibody to detect the activation of Bax. Results The activation of Bax and the decline of NF-κB activity played important roles in the apoptosis of PC12 cells induced by glucose deprivation. Grp75 inhibited the apoptosis induced by glucose deprivation through inhibition of the activation of Bax and the decline of NF-κB activity. There was no change in Bax expression level under glucose deprivation in two groups. Conclusion The activation of Bax and the decline of NF-κB activity were associated with apoptosis of PC12 cells induced by glucose deprivation, and Grp75 provided protection to PC12 cells through inhibition of activation of Bax and maintaining activation of NF-κB.
