Research on histological structure of autofluorescence of Dugesia japonica
10.3969/j.issn.0529-1356.2009.04.024
- VernacularTitle:日本三角涡虫自发荧光组织结构的研究
- Author:
Deming XUE
;
Guangwen CHEN
;
Shangang FU
;
Shuang REN
;
Dezeng LIU
- Publication Type:Journal Article
- Keywords:
Autofluorescence;
Histological structure;
Masson method;
Van Gieson method;
Dugesia japonica
- From:
Acta Anatomica Sinica
2009;40(4):642-646
- CountryChina
- Language:Chinese
-
Abstract:
Objective Hermaphroditic planarians possess a very important position in the systematic evolutionary history of animal, as they are capacity of complete regeneration. Hence, the research on histological structure of autofluorescence has been carried out to provide a crucial insight into the developmental and regenerative biology. Methods Part of histological structure of planarian (Dugesia japonica) was revealed with HE method, Masson method and Van Gieson method. Their autofluorescence was observed with ultraviolet. There were six planarians in each stained group and the autofluorescence group. Results Epidermis, outer epidermis of pharynx, protonephridium, intestine, the photoreceptor cells and longitudinal nerve cords, all radiated blue autofluorescence. The epithelial dissociation side of copulatory bursa radiated yellow autofluorescence, its middle part radiated blue autofluorescence, its fundus side radiated weakly blue autofluorescence. Testis could hardly give off autofluorescence. Pigment cells of eyepot could not give off autofluorescence. Conclusion The research on configuration and autofluorescence of planarian eye may offer help for the study of origin and evolutionary law on eye of invertebrate.
