Immunohistochemical study for the expression of LHR and VEGFon the ovary of mice during peri-implantation
10.3969/j.issn.0529-1356.2009.04.025
- VernacularTitle:围着床期小鼠卵巢黄体生成素受体和血管内皮生长因子表达的免疫组织化学研究
- Author:
Yunzhi SHI
;
Lihua WEI
;
Li GE
;
Hui DU
;
Wengang SONG
- Publication Type:Journal Article
- Keywords:
Luteinizing hormone receptor;
Vascular endothelial growth factor;
Peri-implantation;
Ovary;
Immunohistochemistry;
Mouse
- From:
Acta Anatomica Sinica
2009;40(4):647-650
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological effects of the luteinizing hormone receptor (LHR) and vascular endothelial growth factor (VEGF) on the ovary of mice during peri-implantation. Methods The immunohistochemistry SABC method and image analysis were used to study the distribution and changes of the LHR and VEGF in Kunming mouse( n =28) ovary during estrous,pregnancy of day 1, day 4 and day 6 stage. Results The expression of LHR-immunoreactive substance and that of VEGF-immunoreactive substance had the same distribution and changes. Compared with other groups,the level of LHR-immunoreactive substance and that of VEGF-immunoreactive substance increased highly on the stroma cells around largergrowing follicles in estrous group ( P <0.05). Along with the pregnancy, the positive immunostaining for LHR and VEGF increased gradually on the granulosa lutein cells, and reached the highest level on day 6 of pregnancy. Positive immunostaining for LHR or VEGF on some endothelia and blood cells were observed in day 1 of pregnancy or estrous group respectively. Form day 1 of pregnancy, the theca cells had positive immunostaining for LHR. Conclusion The expression of LHR and VEGF is closely related with the process of follicle growing, ovulation and corpus luteum formation.
