Effect of regional gene therapy with bone morphogenetic protein 7 producing mesenchymal stem cells on spinal fusion in rats

VernacularTitle:骨形态发生蛋白7局部基因治疗和组织工程化骨对大鼠脊柱融合的影响
Author: Lei CHENG ; Lin NIE ; Xin PAN ; Li ZHANG
Publication Type:Journal Article
Keywords: Bone morphogenetic protein 7; Mesenchymal stem cell; Spine fusion; Gene therapy; Western blotting; Rat
From: Acta Anatomica Sinica 2009;40(4):651-655
CountryChina
Language:Chinese
Abstract: Objective To evaluate effectiveness of ex vivo adenoviral gene transfer creating human bone morphogenetic protein 7-producing mesenchymal stem cells (Ad-hBMP-7 cells) compounded with nano-hydroxyapatite/collagen(NHAC) composite to induce spinal fusion in a rat posterolateral spine fusion model. Methods Mesenchymal stem cells were cultured and transduced by Ad-hBMP-7. Then Ad-hBMP-7 cells were cocultured with NHAC. Totally 56 Wistar rats were divided into four groups. Intertransverse spinal arthrodesis was attempted in four groups of Wistar rats with autogenous bone (group A); NHAC(group B); NHAC and BMSCs (group C); Ad-hBMP-7 cells and NHAC (group D). Each specimen underwent plain radiography, manual palpation and histological analysis. Results All spines in group D had fusion. In contrast, none of the spines in group A and group B had fusion. Three of ten rats in group C achieved fusion at the 12th week post-operatively.Conclusion BMP-7-producing mesenchymal stem cells compounded with NHAC composite may induce intertransverse fusion in the rat spine model.