Molecular Cloning of Two Novel Temporins From Lithobates catesbeianus and Studying of Their Antimicrobial Mechanisms
10.3724/SP.J.1206.2009.00033
- VernacularTitle:牛蛙两个新Temporins基因的克隆及其抗菌机制的研究
- Author:
Ruili ZHAO
;
Junyou HAN
;
Wenyu HAN
;
Liancheng LEI
;
Changjiang SUN
;
Xin FENG
;
Lina JIANG
;
Hongwei QIAO
;
Linjun CAI
- Publication Type:Journal Article
- Keywords:
antimicrobial peptides;
Lithobates catesbeianus;
temporins;
transmission electron microscopy
- From:
Progress in Biochemistry and Biophysics
2009;36(8):1064-1070
- CountryChina
- Language:Chinese
-
Abstract:
Temporins are a kind of small,hydrophobic and C-terminus amidated antimicrobial peptides from Rana species.They are effective against bacteria,fungi,yeast,protozoa and viruses.Two novel temporins named as temporin-La(LLRHWKILEKYLanifc) and temporin-Lb(LFRHVVKIFEK.Ylamid.) were cloned from Lithobates catesbeianus.Synthetic peptides of temporin-La and temporin-Lb showed strong antimicrobial activities against bacteria tested,especially Gram-positive bacteria.Besides,temporin-La showed no haemolytic activity to rabbit erythrocytes at the concentration of 250 mg/L while temporin-Lb showed weak haemolytic activity(LC50≈ 230 μmol/L).Transmission electron microscopy showed that temporin-La and temporin-Lb induced different effects on bacterial structure of Staphylococcus aureus.
