Effect of indirubin-3'-monoxime on proliferation and apoptosis of human HT-29 cells
- VernacularTitle:靛玉红甲肟对HT-29细胞增殖和凋亡的影响及机制
- Author:
Xiaobing CHEN
;
Junhui ZHANG
;
Wenjie DONG
;
Xinguang CAO
;
Suxia LUO
;
Zhenhe SUO
- Publication Type:Journal Article
- Keywords:
colon cancer;
indirubin-3'-monoxime;
proliferation;
apoptosis;
survivin;
bcl-2;
Bax
- From:China Oncology
2009;19(7):503-507
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: In recent years indirubin-3'-monoxime has been found to be capable of inhibiting some cell proliferation in vitro and in vivo studies, but human colon cancer HT-29 cells, therefore the purpose in this paper was to study the effect of indirubin-3'-monoxime on proliferation and apoptosis of HT-29 cells and its associated mechanism. Methods: HT-29 cells were treated with indirubin-3'-monoxime. The proliferative status of cells was measured by methabenzthiazuron (MTT) assay, flow cytometry (FCM) was used to measure the apoptosis rate. RT-PCR was used to measure the transcription of apoptosis suppressor gene bcl-2, survivin and apoptosis promoting gene Bar. Results: Indimbin-3'-monoxime inhibited growth of HT-29 cells in a dose-dependent and time-dependent manner (F=11.25, P<0.01). The apoptosis rate increased after the treatment by indirubin-3'-monoxime at 10 μmol/L. There were significant differences between different time groups (F=195.25, P<0.01). The transcription of survivin (F=78.75, P<0.01) and Bax (F=87.61, P<0.01) mRNA in HT-29 cells were increased; the transcription of bcl-2 was significantly decreased (F=95.82, P<0.01). Conclusion: Indirubin-3'-monoxime has obviously inhibited proliferation and induce apoptosis of colon cancer HT-29 cells, its mechanism may be related to decrease the bcl-2/Bax ratio.
