Hydrodynamics-based transgene directively into rat regenerating liver in vivo
10.3969/j.issn.0529-1356.2009.04.016
- VernacularTitle:液压转基因技术应用于大鼠再生肝转基因实验
- Author:
Cunshuan XU
;
Xuekun XING
;
Xianguang YANG
;
Qiushi ZHU
;
Lei DOU
;
Shuaishuai LIU
;
You LI
;
Fuchun ZHANG
- Publication Type:Journal Article
- Keywords:
Liver regeneration;
Hydrodynamics-based transgene;
Gene expression;
Rat
- From:
Acta Anatomica Sinica
2009;40(4):599-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the conditions and methods of hydrodynamics-based transgene into rat regenerating liver in vivo. Methods The solution with concentration 30mg/L gene-containing plasmid was injected into rat tail veins at a speed of 2ml/s, then partial hepatectomy (PH) was performed at different times before/after injection, finally the rat (g) and regenerating liver (g) were weighed, and the liver coefficient (Lc) was calculated. Out of 15 groups which are Lc±Lc*0%, *5%, *10%, *15%, *20%, *25%, *30%, *35%, the most suitable group was chosen as correction coefficient to calculate the most appropriate volume of plasmid solution which was injected into the regenerating liver at different recovery times, and at the same time, right lobe of liver was gathered to make frozen section, then observe and quantify the positive green fluorescent protein (GFP) rate at 488 nm excitation wavelength. Results Injection of either physiological saline or empty plasmid has no significant difference compared with control (only PH performance). The appropriate time of hydrodynamics-based transgene is more than 12 hours before PH or anytime after PH. The solution volume of hydrodynamics-based transgene into liver regenerating rat after PH is rat weight (g) ×9%×1/3×corresponding correction coefficient (Trc). Both vector and target gene have effect on the time and abundance of gene expression. Conclusion Hydrodynamics-based transgene can effectively be applied to gene transfection in rat regenerating liver.
