Analysis of missed detection in gonococci culture of male urethral secretions
10.3760/cma.j.issn.1673-4130.2009.08.015
- VernacularTitle:男性尿道分泌物淋球菌培养漏检情况分析
- Author:
Junwei NIE
;
Guangzhen LIU
;
Zhiyuan LIN
- Publication Type:Journal Article
- Keywords:
Neisseria gonorrhoeae;
Polymerase Chain Reaction;
Male;
Urethra;
Bodily Secretions;
Staining and Labeling
- From:
International Journal of Laboratory Medicine
2009;30(8):762-763
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the undertected situation in gonococci culture of male urethral secretions.
Methods:The 362 cases of male ureethral secreations were simultaneously detected with fluorescence quantitative PCR, germiculture and direct smear method. The results in three detections were statistically analyzed.
Results:The fluorescence quantitative PCR positive rate was 29.56%, near to direct smear method (26.52%)（x2=0.83，P>0.05）; the germiculture positive rate(4.42%) was significantly lower than the positive rates of fluorescence quantitative PCR(x2=81.10,P<0.01) and direct smear method(x2=67.60,P<0.01).
Conclusion:The simple gonococci culture has high missed detection rate(>80%) in detecting male cases. Ensuring the quality of samples before analysis and detecting with two methods or above are the effective measures to improve the gonococci detection rate.
