Identification of novel partner proteins of PCBP1
10.3969/j.issn.1671-167X.2009.04.007
- VernacularTitle:PCBP1的新伴侣蛋白分子的研究
- Author:
Lirong HUO
;
Chen SHEN
;
Weina JU
;
Junhua ZOU
;
Wu YAN
;
W.ted BROWN
;
Nanbert ZHONG
- Publication Type:Journal Article
- Keywords:
Poly(C)-binding protein 1;
Yeasts;
Hybridization,genetic;
Protein binding
- From:
Journal of Peking University(Health Sciences)
2009;41(4):402-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective: PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. We hy-pothesized and were going to identify that the regulatory function of PCBP1 is performed through different complexes of proteins that include PCBP1. Methods: To test our hypothesis, approaches of protein wal-king with a yeast two-hybrid system (Y2H), pulling down in yeasts, co-immunoprecipitation and immu-nofluorescent microscopy assay were employed in this study. The PCBP1 was used as the initial "walker" to search for its interaction partner(s). Results: Candidate proteins including MYL6, PECAM1, CSH1,RAB7, p57KIP2, ACTG1, RBMS1 and PSG4-1ike were identified with selection mediums and preceding methods. Conclusion: With these candidate protein molecules, some protein complexes associating with PCBP1 are proposed, which may help in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1 is involved in variant biological pathways.
