Polymorphism study of six miniSTR loci in Han population from Northeast China
10.3969/j.issn.1673-8225.2009.31.042
- VernacularTitle:中国东北地区汉族群体6个miniSTR基因座多态性
- Author:
Wei MA
;
Zhufeng ZHANG
;
Jian SHEN
;
Yan LI
;
Zifeng PEI
;
Fei XU
- Publication Type:Journal Article
- Keywords:
MiniSTR;
genetic polymorphism;
Han population;
Northeast China
- From:
Chinese Journal of Tissue Engineering Research
2009;13(31):6181-6184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Allele frequencies and forensic parameters for six miniSTR loci(D10S1248,D14S1434,D22S1045,D4S2364,D2S441 and D1S1677)were analyzed in a population of 173 unrelated Chinese Han population individuals from Northeast China.METHODS:The six miniSTR loci were preformed in two multiplex fluorescent PCR systems.ABI 310 Genetic Analyzer was utilized in capillary electrophoresis and the lengths of allele fragments were analyzed.Genetic data collection and analysis software were used for data collection and genotyping.Statistical analysis was performed to the data.RESULTS:The six miniSTR loci showed a moderate degree of polymorphism in Han population from Northeast China.The observed allele sizes were from 67 bp to 115 bp,and the observed heterozygosity ranged from 0.728 to 0.827.The combined power of discrimination and the combined power of exclusion for the six miniSTR loci in Northeast China were 0.999 99,and 0.993 31,respectively.CONCLUSION:The six miniSTR showed a moderate genetic polymorphism in Han population from northeast of China.Due to their small size of PCR amplicon,the six miniSTR could be useful supplements to the CODIS STRs,and they would be useful in population genetics and forensic analysis.
