Influence of sampling errors on ELISA test results
10.3760/cma.j.issn.1673-4130.2009.09.002
- VernacularTitle:ELISA试验加样误差对试验结果的影响分析
- Author:
Hong YUAN
;
Yi MAO
;
Wenfang HUANG
- Publication Type:Journal Article
- Keywords:
Enzyme-linked immunosorbent assay;
Quality control;
Calibration
- From:
International Journal of Laboratory Medicine
2009;30(9):835-837
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of various sampling errors on ELISA test results. Methods Standard sample volume,standard sample volume reducing 1,2,3,4 μL or adding 1, 2,3 μL were respectively pipetted into the wells of a microplate,follwed by routine operation of ELISA test. Then the influence of various sampling errors was analyzed on ELISA test results of HBsAg, HCV and TP. Results S/CO value was increased with the increase of sample volume. The statistical difference of mean S/CO value of HBsAg and TP was only found between sample volume adding 3 μL group and control group(P<0.05). For HCV result, there were significant differences between standard sample volume adding 2,3μL or reducing 3,4μL groups and control group(P<0.05), while no obvious differences were found in the other groups(P>0.05). The difference of mean positive rate between ex-perimental groups and control group showed an increasing tendency with the reduction of sample vol-ume,and significant differences in HBsAg, HCV and TP results were also found between sample vol-ume increase groups and reduction groups(P<0.05). Conclusion Various sampling errors influence ELISA test results to different degrees,and the extent increases with the reduction of standard sample volume.
