Effect of tacrolimus on maturity and allostimulatory activity of cultured dendritic cells of rats in vitro
10.3867/j.issn.1000-3002.2009.05.002
- VernacularTitle:他克莫司对体外培养的大鼠树突状细胞成熟过程及其同种免疫刺激活性的影响
- Author:
Guiming ZHANG
;
Xianghua WANG
;
Lijiang SUN
- Publication Type:Journal Article
- Keywords:
tacrolimus;
dendritic cell;
immune tolerance
- From:
Chinese Journal of Pharmacology and Toxicology
2009;23(5):345-350
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore the effect of tacrolimus on maturity and allostimulatory activity of cultured dendritic cells (DC) in vitro. METHODS Twenty rats were randomly divided into 4 groups: control, tacrolimus, LPS, and tacrolimus+LPS groups. The bone marrow cells of rats were cultured with granulocyte-macrophage colony-stimulating factors and interleukin-4 (IL-4) for 6 d and the DC which adhered to the wall were harvested. No other extraneous reagents were used in control group. Tacrolimus 10 μg·L-1 was added to tacrolimus group at the beginning of culture. Lipopolysaccarides (LPS) 100 μg·L-1 were administered 18 h beforeharvest in LPS group. In tacrolimus+LPS group, tacrolimus and LPS were used in accordance with tacrolimus and LPS groups. The immunophenotypes of DC were analyzed with flow cytometry and the level of IL-12 secreted by DC was detected by ELISA. The allostimulatory activity of DC on allogeneic T cells was assessed with mixed lymphocyte reaction. RESULTS Compared with control group, LPS increased CD80 and CD86 expressions, IL-12 secretion and allostimulatory activities of DC. Compared with control and LPS groups, tacrolimus cut down the expressions of CD80 and CD86, decreased the secretion of IL-12 and reduced the allostimulatory activity of cultured DC. CONCLUSION Tacrolimus exerts a negative effect on the maturation and allostimulatory activity of cultured DC in vitro.
