New bone formation by bone marrow stromal cell combined with the bioactive glass ceramic using tissue-engineering methods
- VernacularTitle:骨髓基质细胞与钙磷陶瓷复合构建组织工程化骨组织
- Author:
Dan JIN
;
Guoxian PEI
;
Qian WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Trauma
2001;17(3):151-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biocompatibility of bioactive glass ceramic (BGC) materials with bone marrow stromal cell (BMSc) and the osteogenic capability of BMSc using tissue-engineering methods. Methods The osteogenic potential in vitro of cultured BMSc in a conditional medium was examined by histochemistry stains technique. The BMSc was cultured in combination with BGC. The attaching and extending speed of the cells to the materials, the proliferation and alkaline phosphatase activity were tested. Then the composite was implanted into the skeletal muscle beds in rabbits. All implants were examined by gross observation and histological examination. Results The BMSc showed a similar property to those of osteoblasts. BMSc can attach to and extend on BGC materials. No inhibition to celluar proliferation and ALP activity were observed by the materials. New bone can be observed in the composites of the BMSc and BGC materials. Conclusions BMSc may provide a rich cellular resource in tissue-engineered bone formation. New bone tissue can be formed by tissue engineering methods.
