δ-opioid receptors protect neurons against neuronal injury induced by oxygen-glucose deprivation
- VernacularTitle:激活δ-阿片受体可对抗原代培养神经元氧糖剥夺损伤
- Author:
Mingwei LI
;
Min ZHU
;
Xuesong TIAN
;
Xiaomin OU
;
Ying XIA
;
Jingchun GUO
- Publication Type:Journal Article
- Keywords:
neuron;
oxygen-glucose deprivation;
δ-opioid receptor;
neuroprotection
- From:
Fudan University Journal of Medical Sciences
2009;36(4):389-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cortical 8-opioid receptor (DOR) on oxygen-glucose deprivation-induced (OGD-induced) neuronal injury. Methods Primary cultured cortical neurons incubated with selective DOR agonist (TAN-67) and antagonist (naltrindole) or PKC inhibitor (chelerythrine, CHE) were exposed to OGD. Lactate dehydrogenase (LDH) release was detected after 24 h reperfusion. The expression levels of DOR were measured by Western blot. Results Compared with OGD group, TAN-67 significantly decreased OGD-indueed LDH release, and increased the expression levels of DOR, while nahrindole aggravated neuronal injury and decreased the DOR protein expression. CHE could abolish the LDH down-regulation induced by TAN-67 plus OGD (P< 0.05, compared with TAN-67 treated group). Conclusions DOR activation protects neurons against OGD injury. PKC might take part in the neuroprotection pathways of DOR.
