Dynamic changes of Kir2.3 mRNA in the hippocampus of rats with chronic temporal lobe epilepsy
- VernacularTitle:慢性颞叶癫痫大鼠海马内向整流钾通道Kir2.3 mRNA的动态变化
- Author:
Lan XU
;
Xunyi WU
;
Zhen HONG
- Publication Type:Journal Article
- Keywords:
temporal lobe epilepsy;
inwardly rectifying potassium channel;
Kir2.3
- From:
Fudan University Journal of Medical Sciences
2009;36(4):427-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe dynamic changes of Kir2. 3 mRNA in the hippocampus of rats with chronic temporal lobe epilepsy, and to discuss the relationship between Kir2. 3 expression and the pathogenesis of chronic temporal lobe epilepsy. Methods We used pilocarpine to induce status epilepticus (SE) in rats,which became chronic temporal lobe epileptic rats in 2 weeks. The expression of Kir2.3 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) at the time points of 0, 6, 72 hours and 2 weeks after SE. Results The ratios of Kir2. 3 mRNA to β-actin of normal control and 0, 6, 72 hours, 2 weeks after SE were 0. 080 ± 0. 030, 0. 103 ± 0. 045, 0. 164 ± 0. 026, 0. 132 ± 0. 024, and 0. 011 ± 0. 008, respectively. The ratio was significantly higher 6 and 72 hours after SE and significantly lower 2 weeks after SE than that of the normal control. Conclusions Two weeks after SE, when the rats had spontaneous recurrent seizures, the expression rate of Kir2.3 reached a turning point, which possibly became the basis of epileptogenesis.
