The effect of receptor tyrosine kinase inhibitor genistein on phenotype expression of collagen and proteoglycan of chondrocyte
- VernacularTitle:三羟基异黄酮对关节软骨细胞合成胶原和蛋白多糖的影响
- Author:
Chao ZHANG
;
Feiyan CHEN
;
Jun XIA
;
Jianyuan JIANG
;
Huangyuan HUANG
- Publication Type:Journal Article
- Keywords:
proteoglycan;
collagen;
receptor tyrosine kinase inhibitor
- From:
Fudan University Journal of Medical Sciences
2009;36(4):440-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective role of the receptor tyrosine kinase inhibitor in chondrocyte in vitro.MethodsReverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry (ICC) and toluidine blue staining were used to understand the changes of phenotype expression of collagenⅠ, Ⅱ and proteoglycan in chondrocyte in vitro. Results Genistein of 20 μg/mL and 25 μg/mL concentrations decreased the expression of collagen Ⅰ and increased the expression of collagen Ⅱ and proteoglycan (P< 0. 05). Conclusions This study showes that genistein can effectively inhibit the expression of collagen Ⅰ above certain concentration, and it can improve the expression of collagen Ⅱ and increase the synthesis of proteoglycan.
