Isolation and preliminary identification of the endophytic fungi which produce Hupzine A from four species in Hupziaceae and determination of Huperzine A by HPLC
- VernacularTitle:4种石杉科植物中产生石杉碱甲的内生真菌的分离及初步鉴定和HPLC测定石杉碱甲的含量
- Author:
Zan JU
;
Jun WANG
;
Shengli PAN
- Publication Type:Journal Article
- Keywords:
Huperzia;
Phlegmariurus;
endophytic fungus;
Hupzine A;
HPLC
- From:
Fudan University Journal of Medical Sciences
2009;36(4):445-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the endophytic fungi which produce Huperzine A from four species in Hupziaceae and improve the culture conditions of screening endophytic fungi. Methods Plant materials were cultivated in culture medium after sterilization and their endophytic fungi were isolated. Hupzine A from metabolic was determined by HPLC, and the strains were identified by microscopic features. Results Thirty-two endophytic fungi were isolated from Huperzia serrata (Thunb. ex Murray) Trev, H. serrata (Thunb. ex Murray) Trev var. longipetiolata (Spring) H. M. Chang, H. appressa (Desv.) Love et D. Love, Phlegmariurus cryptomerianus (Maxim.) Ching ex L. B. Zhang et H. S. Kung. Two of these strains were isolated from P. cryptomerianus, HA15 (Blastomyces sp.) and HA23 (Botrytis sp.), which produced Huperzine A. Conclusions Endophytic fungi producing Hupzine A has been successfully isolated from P. cryptomerianus.
