Content and gene expression of eNOS in heart of hyperlipidemia apoE-/- mice
- VernacularTitle:高脂血症apoE基因敲除小鼠心脏中一氧化氮合酶含量及基因表达
- Author:
Xiaoe CAO
;
Xiaobo LI
;
Lianhua YIN
- Publication Type:Journal Article
- Keywords:
hyperlipidemia;
eNOS;
apoE-/- mice
- From:
Fudan University Journal of Medical Sciences
2009;36(4):403-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the content and gene expression of endothelial nitric-oxide synthase (eNOS) in heart of hyperlipidemia apoE-/- mice. Methods apoE-/- mice were fed with high fat diet as high-fat diet group (n= 7) and normal fat diet as control group (n = 6) for 12 weeks. Serum lipid profiles including total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) were detected, eNOS mRNA expression in heart were determined by Real-Time RT-PCR, as well as protein expression by immunohistochemistry. Results Levels of serum TC, TG and LDL clearly increased in high fat diet group, while HDL decreased (P< 0. 01). mRNA and protein expression of eNOS in heart reduced in high fat diet group(P<0.01). Conclusions High fat diet may lead to hyperlipidemia in apoE-/- mice, which may down-regulate eNOS expression and decrease eNOS content in heart.
