Role of estrogen receptor alpha in adipocytes differentiation of rat bone marrow mesenchymal stem cells

VernacularTitle:雌激素受体alpha对大鼠骨髓基质细胞诱导分化成脂的调控作用
Author: Jing WANG ; Yin DING
Publication Type:Journal Article
Keywords: rBMSC; ER-a; Adipocyte; Transfection; TamoxifenCitrate
From: Journal of Practical Stomatology 2009;25(6):794-797
CountryChina
Language:Chinese
Abstract: Objective; To elucidate the mechanism of the role of ER-a in fat metabolism by regulating the expression of ER-a in SD rats marrow mesenchymal stem cells during differentiating into adipocytes in vitro. Methods ;SD rats marrow mesenchymal stem cells were separated and cultivated. The ER-a was transfected into the rBMSCs in group one. Tamoxifen Citrate was used to restrain the expression of ER-a in group two, the group without any treatment was used as control. Western blot was used to identify the difference of ER-a expression among different groups and Oil-Red-0 staining was employed to identify the adipocytes in vitro. Results; There was significant difference between the number of lipids and different groups (P <0. 01) ,the number of lipid droplet changed concomitantly with ER-a: Low-expression ER-a group > Control group > High-expression ER-a group. Conclusion; ER-a in SD rats marrow mesenchymal stem cells may restrain the adipocyte differentiation.