Effect of short exposure to high concentrations of fluoride on the expression of DSP in odontoblasts of mice molars
10.3969/j.issn.1001-3733.2009.06.12
- VernacularTitle:短期高浓度氟对小鼠磨牙胚中牙本质涎蛋白表达的影响
- Author:
Jing ZHANG
;
Zhangzhang JI
;
Lingxuan MEI
- Publication Type:Journal Article
- Keywords:
Fluoride;
Odontoblasts;
DSP
- From:
Journal of Practical Stomatology
2009;25(6):816-819
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of short exposure to high levels of fluoride on cellular structure and the DSP expression of odontoblasts of mice molars. Methods: 32 4-day-old ICR mice were randomly divided into two groups. The experimental animals were received a single intraperitoneal dose of 20 mg(n =8) andlO(n=8) mg NaF/kg( body weight) respectively. Equal doses of NaCl were given to the controls (n = 8 for each group). The injected volume was kept constant (10 μ/g). After 24 hours all mice were sacrificed. HE staining and immunohistochemical staining were used to observe the structural changes and the expression of DSP in odontoblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the results. Results: The secretory odontoblasts distorted and lost its normal column contour. The immunohistochemistry results demonstrated that the expression of DSP was more intense than that in control group. Results of statistics analysis showed there were significant differences between the two groups(P<0.01). No remarkable differences were found in mature odontoblasts. Conclusion: Short exposure to high concentration of fluoride can enhance the expression of DSP in the secretory odontoblasts,and inhibit differentiation of the odontoblasts and matrix secretion. This lead to the abnormal development of dentinogenesis.
