Effect of hypoxia on adhesion and invasion of human tongue squamous cell carcinoma cell line Tca8113
10.3969/j.issn.1001-3733.2009.06.15
- VernacularTitle:乏氧对人舌鳞癌细胞系Tca8113体外黏附和侵袭能力的影响
- Author:
Ying SONG
;
Shanzhen SUN
;
Xun QU
;
Wenxia WANG
;
Xiaoying ZHANG
;
Bao SONG
- Publication Type:Journal Article
- Keywords:
Oral carcinoma;
Neoplasm metastasis;
HIF-1;
Gene silencing
- From:
Journal of Practical Stomatology
2009;25(6):828-832
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the effect of synthesized small interfering RNA targeting to HIF-lα on the adhesion and invasion of human tongue squamous cell carcinoma cell line (Tca8113). Methods; A double strand small interference RNA (siRNA) targeting HIF-1α (siRNAH1Fla) was transfected into cultured Tca8113 cells by lipofectamine2000. The expression of HIF-1α was investigated on mRNA level by real time-PCR and protein level by Western blot. The adhesion and invasion of Tca8113 cells to extracellular matrix (ECM) was also analyzed. Results: Exposure to hypoxia induced a prolonged elevation of HIF-lα protein and siRNAHIF.la reduced HIF-la synthesis as measured on mRNA level and protein level compared with the controls. No matter under normoxic or hy-poxic conditions, the adhesion potency of siRNAHIF-1α treated Tca8113 cells was markedly inhibited compared with controls(P<0.05 or P <0.01). So did the invasion potency (P<0.01). The adhesion and invasion potency of siRNAHIF.,a treated Tca8113 cells were inhibited more greatly under hypoxic condition than under normoxic condition ((36.4±2.7)% vs(26±2.35);(44.2±2.2)% vs (35±1.75), P<0.01)). Conclusion; siRNAH1F.lo can knockdown the expression of HIF-la and inhibit the cell adhesion and invasion to ECM in Tca8113 cells. HIF-la may play an established role in the regulation of Tca8113 cells invasion and metastasis. Interfering with HIF-1α pathways by siRNA strategy may provide a therapeutic target for human tongue squamous cell carcinomas.
