An experimental study on cytotoxicity of monocytes/macrophages against Tca8113 cells in hypoxic microenvironment
10.3969/j.issn.1001-3733.2009.06.18
- VernacularTitle:单核/巨噬细胞在缺氧微环境下对Tca8113舌鳞癌细胞系杀伤作用的实验研究
- Author:
Hongchao FENG
;
Jiangfan PENG
;
Hong MA
;
Yufeng SONG
;
Lu TANG
- Publication Type:Journal Article
- Keywords:
Monocytes/Mcrophages;
Tongue;
Squamous cell carcinoma;
Hypoxia;
Killing activity
- From:
Journal of Practical Stomatology
2009;25(6):842-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective; To investigate the cytotoxicity of monocytes/macrophages against Tca8113 cells in hypoxic microenvironment in vitro. Methods; Peripheral blood mononuclear cells were extracted from healthy person^ blood and cultivated to transform into monocytes/macrophages. The monocytes/macrophages were cultured with Tca8113 cells in hypoxic microenvironment (O_2 < 1%) and in normal microenvironment (20% O_2). The cytotoxicity of monocytes/macrophages against Tca8113 cell was examined by MTT assay. Results;The cytotoxicity of monocyles/macrophages against Tca8113 cells in hypoxic microenvironment was lower than that in normal environment(P<0.05). Conclusion; In hypoxic microenvironment, the cytotoxicity of monocytes/macrophages against Tca8113 cells is decreased. Bui the mechanism of monocytes/macrophages' function in tumors need further research.
