Effect of hyperthermia in combination with progresterone on a human tongue cancer drug-resistance cell line Tca8113/BLM

VernacularTitle:加热联合黄体酮对人舌癌耐药细胞株Tca8113/BLM耐药性的影响
Author: Chunmei HE ; Dilan QIN ; Ke DENG
Publication Type:Journal Article
Keywords: Hyperthermia; Prog resterone; TcaSl 13/BLM; P-gp; MRP
From: Journal of Practical Stomatology 2009;25(6):862-864
CountryChina
Language:Chinese
Abstract: Objective; To explore the possible effect of hyperthermia combined with Prog on a human tongue cancer cell line Tca8113 and drug-resistance cell line Tca8113/BLM in vitro. Methods;The effect of hyperthermia combined with Prog on Tca8113 and Tca8113/ BLM cells were determined by MTT. The concentration of ADM, the expression of P-gp and MRP were detected by flow cytometry (FCM). Results;Compared with Prog or hyperthermia, the hyperthermia at 41 X. for 1 h combined with Prog showed obvious inhibitory effect in Tca8113 and Tca8113/BLM cells(P<0.01). The concentration of ADM in Tca8113 and Tca8113/BLM cells increased obvi-ously(P<0.01). Expression levels of P-gp and MRP in Tca8113 and Tca8113/BLM cells decreased significantly. Conclusion:The hyperthermia combined with Prog can increase the intracellular concentration of ADM in Tca8113 and Tca8113 /BLM cells, which reverse the drug-resistance of Tca8113/BLM cells to BLM and down-regulate expressions of P-gp and MRP.