Effects of PI3-K/AKT signal transduction pathway in rat neural stem cells
- VernacularTitle:大鼠神经干细胞中PI3-K/AKT信号转导通路的研究
- Author:
Yu ZHAO
;
Peng XIE
;
Xiaofeng ZHU
- Publication Type:Journal Article
- Keywords:
phosphatidylinositol 3 kinase;
protein kinase B;
neural stem cells,rat
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2009;29(10):1191-1195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To incubate rat neural stem cells (NSCs) with selection-specific inhibitor ( LY294002) of PI3-K, and explore the effects of PI3-K/AKT signal transduction pathway in NSCs. Methods NSCs derived from E15-16 rats were isolated and cultured. After incubation of NSCs with different concentrations of LY294002 (0 to 40 μmol/L), cell survival ( WTS-8 assay), proliferation ( BrdU immunohistochemical identification), differentiation (β Tubulin-III immunohistochemical identification) and AKP phosphorylated protein (Western blotting) were detected. Results The effects of LY294002 on the survival, proliferation and differentiation of NSCs was concentration-dependent. The survival of the NSCs decreased significantly with a higher concentrations of LY294002 (25, 30, 35, 40 μmol/L) (P < 0. 05). The numbers of BrdU positive cells with LY294002 of 30, 35 and 40 μmol/L were significantly smaller than that of the control (LY294002 0 μmol/L) (P < 0.05). The numbers of β Tubulin-III positive cells with LY294002 of 35 and 40 μmol/L were significantly smaller than that of the control (P < 0. 05). The expression of AKT was blocked by LY294002 in a concentration-dependent manner. Conclusion PI3-K/AKT signal transduction pathway plays a vital role in the survival, proliferation and differentiation of NSCs.
