ERCC2 expression in siRNA interference of esophageal cancer cells and correlation with paclitaxel sensitivity
- VernacularTitle:siRNA干扰ERCC2表达对食管癌细胞紫杉醇敏感性的影响
- Author:
Yudong WANG
;
Zhiying CUI
;
Jing ZUO
;
Li FENG
;
Yalei LV
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
esophageal carcinoma cell lines;
ERCC2;
taxol;
IC_(50);
siRNA
- From:
China Oncology
2009;19(10):761-765
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: ERCC2 gene silence by siRNA interference was observed in esophageal cancer cell line and it could change cell sensitivity to taxol. This study was to investigate the biological mechanism of paclitaxel-resistance in esophageal cancer cells. Methods: ERCC2 targeting siRNA (si-ERCC2) has been synthesized. The constructor were transfected into ERCC2 cell lines high-KYSE150(bigh expression of ERCC2) through lipofectamine. RT-PCR and flow cytometry (FCM) were used to detect the ERCC2 mRNA and protein expression levels. MTr assay was used to estimate the paclitaxel sensitivity of the cells. Results: In si-ERCC2 group, ERCC2 could not be detected and ERCC2 protein expression were reduced by 31.2%, 51.6% and 60.0%, respectively, 24,48,72 b after transfection. Paclitaxel IC_(50) value for si-ERCC2 group was 6.32±0.87 μg/mL, lower than the control group(49%). Conclusion: siRNA could successfully silence the target gene ERCC2 at the level of transcription and translation of the gene, the reduction of ERCC2 expression may reverse taxol resistance of the cells.