Inhibitory effects of survivin antisense oligonucleotide on human endometrial carcinoma cells
- VernacularTitle:生存素反义寡核苷酸对人子宫内膜癌细胞增殖的抑制作用
- Author:
Jinyu XU
;
Yaping CHEN
;
Bingjin CHEN
- Publication Type:Journal Article
- Keywords:
antisense oligonucleotide;
survivin;
endometrial cancer cells
- From:
Fudan University Journal of Medical Sciences
2009;36(6):723-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of survivin antisense oligonucleotide (ASODN) on human uterine endometrial cancer HEC-1B cell line. Methods The experiment included four groups: blank control group (blank), lipofectin control group (Lip), oligonucleotide (SODN) control group and antisense oligonucleotide (ASODN) test group. Artificially synthesized survivin antisense oligonucleotide was transfected for 48 hours into endometrial cancer cells using liposome after cultivation. The expression of survivin protein was determined by Western blot, and the cellular apoptotic rate was tested by flow cytometric analysis. MTT method was used to assay cellular growth rate. Results In comparing with the blank, Lip and SODN control groups, survivin protein expression was obviously decreased in HEC-1B cancer cells after transfected with survivin ASODN. The apoptotic rate and proliferation inhibition rate in ASODN test group were significantly higher than that in the control groups (P<0.05). There was no significant difference between other control groups (P>0.05). Conclusions The expression of survivin is decreased in endometrial cancer cells after ASODN transfection. Survivin ASODN causes cellular apoptosis and inhibits the proliferation of HEC-1B cells. It may be a useful gene therapy for endometrial cancer.
