Induction of apoptosis by Anti-DR5 mAb in synovial cells of adjuvant arthritis rats and its possible mechanisms
- VernacularTitle:Anti-DR5 mAb诱导佐剂型关节炎大鼠滑膜细胞凋亡及机理初探
- Author:
Wenzhu LI
;
Ping YIN
;
Jiakai ZHANG
;
Jinhua QIU
;
Qingzhong HU
;
Huiran TAO
;
Guohong ZHUANG
- Publication Type:Journal Article
- Keywords:
Anti-DR5 mAb;
Adjuvant arthritis;
Synovial cells;
Cell apoptosis
- From:
Chinese Journal of Immunology
2009;25(11):969-972
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Anti-DR5 mAb on inducing synovial cells of adjuvant arthritis (AA) rats and its mechanisms.Methods:AA was induced by CFA in rats.The synovial cells of rats were separated and cultured in vitro system.The apoptosis induced by anti-DR5 mAb on synovial cells was measured by MTT analysis,DNA fragmentation and flow cytometry.Caspase-3 and Bcl-2 expression in synovial cells were detected by Western blot.The involvement of the apoptotic pathway was further proved by a caspase inhibition assay.Results:MTT result showed that Anti-DR5 mAb could inhibit synovial cells growth.And flow cytometry suggested that the cell death mode was apoptosis.The protein level of caspase-3 in synovial cells treated with anti-DR5 mAb was raised,while Bcl-2 level declined.When the caspase inhibitor was added to the synovial cells treated with anti-DR5 mAb,it was showed in a dose-dependence inhibition effect,indicating that anti-DR5 mAb inducing apoptosis might be through the caspase pathway.Conclusion:Anti-DR5 mAb could induce synovial cell apoptosis through caspase pathway.And this effect may be related to the protein level of Caspase-3 and Bcl-2.
