Significance and changes of serum interleukin - 8 and 10 in human myocardium ischemic preconditioning
- VernacularTitle:血清白细胞介素8和白细胞介素10在心肌缺血预处理中的变化及意义
- Author:
Guangxian CHEN
;
Zhongkai WU
;
Baiyun TANG
;
Hai LIU
;
Xi ZHANG
- Publication Type:Journal Article
- Keywords:
Ischemic preconditioning;
Proein Bel-2;
Caspase-3;
Cytokines
- From:
Chinese Journal of Pathophysiology
2009;25(11):2088-2092
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the mechanism of myocardium protection after ischemia/reperfusion (I/R) injury by preconditioning with ischemia in human. METHODS: Thirty - six patients underwent valve replacement were divided into ischemic preconditioning group (IP group, 20 cases) and non -ischemic preconditioning group (control group, 16 cases) according to whether they were given single cycle reperfusion before cardioplegia or not. Serum levels of interleukin -8 and 10 were measured with ELISA. Expressions of myocardial Bel -2 and caspase -3 were analyzed. RESULTS: The inflammatory factors IL - 8 and IL - 10 increased to the highest level in serum at 6 h after declamping and recovered to normal level on 5 d after declamping. On 6 h, 1 d and 2 d after declamping, serum level of IL -8 was significantly lower in IP group than that in control group ( P < 0.05 ) , but serum level of IL -10 was higher in IP group (P < 0.05 ). Expression of myocardial Bel - 2 and caspase - 3 increased in both groups after reperfusion, and Bel - 2 was lower in the control group than that in IP group while the level of caspase - 3 was higher (P < 0.05). Expression of myocardial Bel - 2 had positive correlation with IL - 10 and negative correlation with IL - 8.CONCLUSION: Ischemic preconditioning has the effect of protection of human myocardial cells after ischemia/reperfusion injury through decreasing systemic inflammatory response following ischemia reperfusion injury.
