The changes and significance of pulmonary vascular endothelial cell,ICAM-1 and E-selectin in sepsis rats
- VernacularTitle:脓毒症大鼠肺血管内皮细胞、细胞间粘附分子1和E-选择素的变化及其意义
- Author:
Zhijie HE
;
Zijun ZOU
- Publication Type:Journal Article
- Keywords:
Sepsis;
Vascular endothelial cell;
ICAM-1;
E-selectin
- From:
Chinese Journal of Immunology
2009;25(11):1029-1032
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes and significance of pulmonary vascular endothelial cells (VEC),ICAM-1 and E-selectin in sepsis rats.Methods:60 SD rats were randomly divided into 2 groups:the control group and the sepsis group.The sepsis model was prepared by injection of lipopolysaccharide(4 mg/kg).The expression of ICAM-1 and E-selectin in pulmonary VEC of rats was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical method.The VEC apoptosis in lung was analyzed with Hoechest-33258 staining.The ultramicrostructure of pulmonary VEC was observed under electron microscope.Results:Compared with the control group,the expression of ICAM-1 was significantly increased in the sepsis group (P<0.01),the expression of ICAM-1 was increased gradually and achieved the peak value at 24 h.The expression of E-selectin was achieved the peak value at 6 h and decreased gradually during 24 h.The apoptosis and necrosis of pulmonary VEC was increased gradually and achieved the peak value at 24 h (P<0.01).Conclusion:The expression of ICAM-1 and E-selectin in sepsis rats is significantly increased,probably leading to both necrosis and apoptosis of pulmonary VEC,resulting in the occurrence of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).
