Effects of perfluorochemical on in vitro long-term hypothermic heart preservation in rats
- VernacularTitle:全氟碳化合物在离体心脏长时程低温保存中的作用
- Author:
Jie XIANG
;
Zhoubin LI
;
Wangfu ZANG
- Publication Type:Journal Article
- Keywords:
perfluorochemical;
two-layer method;
heart preservation;
energy metabolism;
rat
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2009;29(11):1331-1335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of perfluorochemical ( PFC) on in vitro long-term hypothermic heart preservation in rats. Methods Thirty Wistar rats were randomly divided into control group ( Celsior solution), Celsior + O_2 group (Celsior solution plus oxygen) and PFC/Celsior + O_2 group (two-layer method plus oxygen) , with 10 rats in each group. Langendorff model of isolated rat heart was prepared. The isolated heart was preserved at 4 ℃ for 8 h, and hemodynamic parameters, coronary effluent flow, and leakage of creatine kinase ( CK), lactate dehydrogenase ( LDH) and aspartate transaminase (AST) were detected after reperfusion. Besides, the myocardial ultrastructure was also observed. Results Compared with control group and Celsior + O_2 group, the left ventricular developed pressure and ± dp/dt in PFC/ Celsior + O_2 group significantly increased ( P < 0.01), while LDH, CK and AST leakage significantly decreased ( P < 0.01). However, there was no significant difference in the above paramters between Celsior + O_2 group and control group (P>0.05). Compared with control group and Celsior + O_2 group, the impairment of myocardial ultrastructure in PFC/ Celsior + O_2 group after hypothermic preservation was alleviated. Conclusion PFC as a supplementation to oxygen in heart preservation solution could enhance myocardial protection during in vitro long-term hypothermic heart preservation in rats via the improvement of energy metabolism.
