Mutations of WNK gene in patients with hypokalemic salt-losing tubulopathies
- VernacularTitle:低钾失盐性肾小管病患者WNK基因突变研究
- Author:
Chong ZHANG
;
Ling QIN
;
Leping SHAO
;
Zhaohui WANG
;
Weiming WANG
;
Hong REN
;
Wen ZHANG
;
Fuhong YAN
;
Jingyuan XIE
;
Nan CHEN
- Publication Type:Journal Article
- Keywords:
hypokalemic salt-losing tubulopathies;
WNK kinase;
mutation;
sequencing
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2009;29(11):1344-1350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanisms involved in hypokalemic salt-losing tubulopathies ( SLTs) through genetic screening of WNK gene in patients with SLTs. Methods Forty-four kindreds of SLTs were diagnosed Batter's syndrome or Gitelman's syndrome after CLCNKB and SLC12A3 sequencing and analysis, 8 of whose phenotype can not be simply attributed to CLCNKB or SLC12A3 mutations. Primers for PCR-amplified exons of WNK4 and WNK1 gene in genomic DNA were designed, and direct sequencing was performed to analyse the PCR products. Results Two missense mutations of WNK1, Ile~(1172)→ Met (I1172M) and Ser~(2047) → Asn (S2047N), were identified. Both of these 2 mutations segregated with the disease in SLTs kindred. Conclusion Two heterozygote missense mutations of WNK1 gene (I1172 M and S2047N) were found in 8 SLTs kindreds, indicating that WNK1 might be another gene responsible for hypokalemic salt-losing tubulopathies.
