Progress in The Formation and Transfer of Agrobacterium T-complex
10.3724/SP.J.1206.2009.00195
- VernacularTitle:农杆菌T-复合物的形成与转运研究进展
- Author:
Minliang GUO
;
Diankun GAO
;
Yingshan JIN
- Publication Type:Journal Article
- Keywords:
Agrobacterium tumefaciens;
T-complex;
type Ⅳ secretion system;
T-DNA transfer
- From:
Progress in Biochemistry and Biophysics
2009;36(11):1408-1414
- CountryChina
- Language:Chinese
-
Abstract:
Agrobacterium tumefaciens can transfer a DNA fragment (T-DNA) from its Ti plasmid to host plant and integrate the T-DNA fragment into host cell nuclear genome. Agrobacterium-mediated T-DNA transfer is the most widely used genetic transformation method for plant. The T-DNA is delivered in the form of single-stranded T-DNA-protein complex (T-complex) by the polar-located Agrobacterium type Ⅳ secretion system (T4SS) to the host cell. T4SS is ancestrally related to bacterial conjugation machines and still used by many plasmids as conjugation channel. Moreover, T4SS is also the secretion channel that used by many human bacterial pathogens to inject the effector proteins to host cells, thus contributing directly to the bacterial pathogenicity. Therefore, in addition to the technical application as a gene vector to create transgenic plants, Agrobacterium-mediated T-DNA transfer also provides a fascinating model system to study the intercellular transfer of macromolecules. The study on the molecular mechanism of T-DNA transfer arouses extensive interest and progresses rapidly in recent years. Here the recent advances in research on T-complex formation and T-complex transfer in Agrobacterium cell are reviewed.
