In vitro Efficacy of mda-7 Gene for Hepatocellular Carcinoma Gene Therapy Mediated by Human Ribosomal DNA Targeting Vector
10.3724/SP.J.1206.2009.00255
- VernacularTitle:人核糖体DNA打靶载体介导mda-7基因对肝癌的体外基因治疗研究
- Author:
Jinfeng XUE
;
Xionghao LIU
;
Qiang HE
;
Zhigang XUE
;
Youjin HI
;
Zhuo LI
;
Junlin YANG
;
Ting GAO
;
Qian PAN
;
Zhigao LONG
;
Lingqian WU
;
Kun XIA
;
Desheng LIANG
;
Jiahui XIA
- Publication Type:Journal Article
- Keywords:
hrDNA targeting vector;
mda-7 gene;
hepatocellular carcinoma;
gene therapy
- From:
Progress in Biochemistry and Biophysics
2009;36(11):1429-1435
- CountryChina
- Language:Chinese
-
Abstract:
Human ribosomal DNA (hrDNA) targeting vector pHr is a homologous recombinant plasmid for human genome which developed in the State Key Laboratory of Medical Genetics. pHr was used to construct a recombinant plasmid pHr-CMG expressing mda-7/GFP fusion gene and its efficacy in the hepatocellular carcinoma cell line Bel-7402 was investigated. The expression of mda-7/GFP fusion gene was detected by fluorescent microscope, RT-PCR and Western blotting, and its function was detected by cell-cycle analyses, MTT assay and Hoechst33258 staining. The results demonstrated that pHr-CMG vector could express MDA-7/GFP fusion protein effectually and the mda-7 gene could induce cell apoptosis and proliferation suppression in Bel-7402 cell line, which might be caused by the G2/M cell cycle arrest. These results also suggested that human ribosomal DNA targeting vector system and the pHr-CMG vector may be applied in further gene therapy researches for hepatocellular carcinoma.
