Using FRET to Study The Interaction Domain of TLR4 Binding to MD-2 in Living Cells
10.3724/SP.J.1206.2009.00246
- VernacularTitle:利用荧光共振能量转移技术研究活细胞TLR4与MD-2作用结构域
- Author:
Tianyu ZHONG
;
Jing TANG
;
Dengyu CHEN
;
Yawei LIU
;
Wei WANG
;
Jinghua LIU
;
Yong JIANG
- Publication Type:Journal Article
- Keywords:
MD-2;
TLR4;
LPS;
fluorescence resonance energy tramsfer(FRET);
signal transduction
- From:
Progress in Biochemistry and Biophysics
2009;36(11):1451-1457
- CountryChina
- Language:Chinese
-
Abstract:
TLR4-MD-2 complex plays a key role in LPS recognition and its signal transduction. These steps are the vital elements of the host's defensive reaction. Studying the functional domain of TLR4 and MD-2 is very important to further understand the mechanism of LPS signal transduction. It was studied the interaction domain of TLR4 and MD-2 in living cells based on gene mutation, gene transfection and fluorescence resonance energy tramsfer(FRET) which is considered as one of the best methods used for intracellular protein-protein interaction study. CY-15P which was fused by CFP and YFP through 15 neutral amino acids was used as positive control, while co-expressed CFP and YFP proteins were used as negative control. The results showed that the ability of TLR4 binding to MD-2 decreased dramatically after the deletion of Glu~(24) ～ Met~(41) in N terminal of TLR4. Aggregation of TLR4 to LPS stimulation was observed, however, TLR4 without the Glu~(24)～ Met~(41) mutation did not aggregate. All these results indicated that TLR4 Glu~(24)～ Met~(41) might be the interaction domain of TLR4 binding to MD-2 and participate in the aggregation effect of TLR4 upon LPS stimulation.
