The Effects of BHC80 Down Regulation on Embryonic Erythropoiesis in Zebrafish
10.3724/SP.J.1206.2009.00301
- VernacularTitle:BHC80基因抑制对斑马鱼胚胎红细胞发育的影响
- Author:
Jiayun HOU
;
Qiu JIANG
;
Jie ZHANG
;
Li TAN
;
Houyan SONG
- Publication Type:Journal Article
- Keywords:
zebrafish;
BHC80;
erythropoiesis;
in situ hybridization
- From:
Progress in Biochemistry and Biophysics
2009;36(11):1469-1474
- CountryChina
- Language:Chinese
-
Abstract:
Whole mount in situ hybridization with BHC80 RNA probe showed that BHC80 was expressed in zebrafish central nervous system. Morpholino-modified antisense oligonucleotide was injected into zebrafish zygotes to knock down BHC80 expression. BHC80 knockdown resulted in striking decrease of erythrocytes and accumulation of erythrocytes at PBI. Further investigation of embryonic erythrocytes marker βe3 globin and hematopoiesis transcription factors gata1, c-myb and lmo2 by in situ hybridization showed that the erythroid progenitors marked with gata1 in BHC80 knockdown embryos were high proliferation and their differentiation were delayed, which led to decrease of erythrocytes and accumulation of erythrocytes at PBI. Both in situ hybridization and microangiography indicated that vasculature pattern of BHC80 knockdown embryos were almost normal.
