Effects of apolipoprotein (a) on vascular smooth muscle cells proliferation and cell signal transduction pathway
- VernacularTitle:载脂蛋白(a)对血管平滑肌细胞增殖及其信号通路的影响
- Author:
Chunying WEI
;
Menghong WANG
;
Yunfeng WEI
;
Zeqi ZHENG
;
Jingtian PENG
;
Jun HUANG
;
Yuan WEN
;
Zhiyong WU
- Publication Type:Journal Article
- Keywords:
Apolipoproteins A;
Vascular smooth muscle cells;
Cell proliferation;
Transforming growth factor beta
- From:
Chinese Journal of Pathophysiology
2009;25(11):2122-2125
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect and the mechanism of apolipoprotein (a) [apo (a) ] on proliferation of vascular smooth muscle cells ( VSMCs). METHODS: All VSMCs used in experiments were serial subcultured from primary cells and were identified by immunohistochemistry staining of a - actin. Cell growth assay was observed as cell counting and MTT assay. Western blotting was also employed to detect the related mechanism. RESULTS: All cells used in experiments were confirmed as VSMCs. Although apo (a) enhanced VSMCs proliferation, this effect was attenuated by anti -integrin α_vβ_3, LM609.Use these reagents alone had no effect on VSMCs growth. The results of Western blotting demonstrated that focal adhesion kinase (FAK) was activated by apo (a) and the expression of total or phosphorylated transforming growth factor β_1 (TGF -β_1) was also decreased. However, these effects described above were all blocked by LM609.CONCLUSION: Apolipoprotein (a) enhances VSMCs proliferation and this effect is mediated by integrin α_vβ_3, which activates FAK and attenuates TGF - β_1 and phospho -TGF - β_1 expression.
