Cellular localization of BS69 and the identification of its functional nuclear export signal sequence
- VernacularTitle:BS69的亚细胞定位及其核输出信号序列的分析鉴定
- Author:
Chao ZHANG
;
Bo YU
;
Yong SHAO
;
Hong YANG
;
Wei ZHANG
;
Jun WAN
- Publication Type:Journal Article
- Keywords:
Adenovirus EIA - associated protein BS69;
Nuclear export signal sequence;
Cellular localization;
Transcription factors
- From:
Chinese Journal of Pathophysiology
2009;25(11):2145-2149
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify the novel nuclear export signal by analyzing the DNA sequences and detecting the cell localization of different adenovirus ElA - associated protein BS69 isoforms. METHODS:BS69 DNA sequences in Emsebl database were blasted and the sequence of amino acids was aligned with the typical nuclear export signal. Different BS69 isoform fragments were cloned into pcDNA3.1 vector and transfected into Cos7 cells. The BS69 localization was observed by immunostaining and the function was verified by Western blotting. RESULTS: A novel nuclear export signal was found in BS69 isoform 2 but not in isoform 1.The isoform 2 was localized in cytoplasm and isoform 1 in nucleus, which was also consistent with the DNA sequence. The isoform 2 was involved in LMP1 - activated JNK phosphorylation whereas the isoform 1 was not. CONCLUSION: Different BS69 isoforms have different cellular localization. BS69 isoform 2 is localized in cytoplasm, interacting with Epstein - Barr virus latent membrane protein 1 and may be involved in nasopharyngeal carcinoma development.However, the isoform 1 is localized in nucleus and plays important roles in transcription.
