Effects of MT1- MMP on collagen metabolism regulated by FRNK in hepatic stellate cells
- VernacularTitle:膜型基质金属蛋白酶-1在黏着斑激酶相关非激酶调控肝星状细胞胶原代谢中的作用
- Author:
Juan WEI
;
Xiaolan ZHANG
;
Zhina DUN
;
Chunhong ZHAO
;
Jiangang SHEN
;
Xiaoxia HUO
;
Junyan AN
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cells;
Focal adhesion kinase;
Focal adhesion kinase -related non - kinase;
Membrane - type matrix metalloproteinase - 1;
Collagen;
Liver cirrhosis
- From:
Chinese Journal of Pathophysiology
2009;25(11):2155-2158
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of FAK - related non - kinase ( FRNK) on the expression of membrane - type matrix metalloproteinase -1 ( MT1 - MMP) in hepatic stellate cells ( HSC). METHODS:FRNK were trans-fected into HSCs by cationic liposome method. The protein levels of FRNK in HSC were assayed by Western blotting. The levels of MT1 - MMP were determined by RT - PCR for mRNA and by Western blotting for protein, respectively. RESULTS: The up -regulated expression of FRNK protein was observed and it was at 48 h after transfection that the FRNK protein content was the highest ( P < 0.05 ). The expressions of MT1 - MMP mRNA and protein were also up - regulated by the transfection of FRNK, and it was at 48 h after transfection that the MT1 - MMP protein content was significantly increased. CONCLUSION: The mRNA and protein of FRNK were over - expressed in HSC transfected with the gene of FRNK. The inhibitory effect of FRNK on the collagen synthesis in HSC may be through the up - regulation of MT1 - MMP.
