Effects of ginkgolide B on [ Ca~(2+) ]_i and mitochondrial function of cultured rat retinal neurons in vitro
- VernacularTitle:银杏内酯B对体外培养的视网膜神经细胞内钙离子浓度和线粒体功能的影响
- Author:
Jing MENG
;
Xiaoyan DING
;
Xiaobo ZHU
;
Shaofen LIN
;
Mengxiang GUO
;
Shibo TANG
- Publication Type:Journal Article
- Keywords:
Ginkgolide B;
Glutamic acid;
Intracellular calcium ion concentration;
Mitochondrial membrane potential;
Retina
- From:
Chinese Journal of Pathophysiology
2009;25(11):2192-2196
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of ginkgolide B (CB) on the intracellular calcium ion concentration ( [ Ca~(2+) ]_i) and mitochondrial function of cultured rat retinal neurons in vitro. METHODS: in vitro primary culture of rat retinal neurons was used in the experiment. The apoptosis model of glutamate - induced retinal neurons was established and co - cultured with ginkgolide B. The [ Ca~(2+) ]_i and mitochondrial membrane potential of the retinal neurons were detected by laser scanning confocal microscope. RESULTS: Glutamate decreased the survival rate of retinal neurons, increased the apoptosis and the [ Ca~(2+) ]_i, lowered the mitochondrial membrane potential. The [ Ca~(2+) ]_i was clearly diminished and the mitochondrial membrane potential was significantly increased with the GB intervention, and the apoptosis decreased significantly. CONCLUSION: GB protects retinal neurons from glutamate induced neurotoxicity. The effect of GB on retinal neurons might be due to its ability to decrease the [Ca~(2+) ]_i and increase mitochondrial membrane potential.
